Development and application of oligonucleotide microarray for detecting rifampin - resistant mycobacterium tuberculosis strains 應(yīng)用微孔噬菌體擴增法檢測結(jié)核分枝桿菌對利福平的敏感性
Reliable detection of rifampin - resistance of mycobacterium tuberculosis strains by using a specialized oligonucleotide microarray 檢測耐利福平結(jié)核分枝桿菌寡核苷酸芯片的研制及應(yīng)用
Oligonucleotide microarray technology is a powerful data - mining platform and has been widely applied in biosciences 摘要寡核苷酸芯片技術(shù)是一種高通量發(fā)掘和采集生物信息的強大技術(shù)平合,目前已廣泛應(yīng)用于生物科學(xué)領(lǐng)域。
On the basis of the first step of amplifying pcr , it has been hybridized with oligonucleotide microarray in order to analyze whether the amplified pcr product possess antimicrobial agent resistance or not 摘要在第一步耐藥性靶基因得到擴增的基礎(chǔ)上,通過與芯片進行雜交來分析所擴增的pcr產(chǎn)物是否是已經(jīng)發(fā)生了突變而能導(dǎo)致抗藥性的基因。
To improve the performance of assays on the oligonucleotide microarray , the factors that influence the hybridization effects such as surface chemistry , probe size , spacer length , hybridization conditions etc were intensely studied and optimized 為改善寡核苷酸芯片的分析性能,對影響芯片雜交結(jié)果的因素,如片基表面的化學(xué)處理、探針的長度、間隔臂的長度、雜交條件等,進行了深入的研究和優(yōu)化。
oligonucleotide microarray meaning:[Medicine] Hybridization of a nucleic acid sample to a very large set of oligonucleotide probes,which are attached to a solid support,to determine sequence or to detect variations in a gene sequence o...